Snippets from My Master’s Journey

Sat, 30 May 2026 00:00:00 +0000

Throughout my master’s journey, I have been fortunate to grow academically and professionally. This page shares selected moments that shaped this experience.

During the pre-master’s year, I completed 10 courses (30 credit hours) and contributed to curriculum preparation and presentations delivered to my colleagues.

By the end of the year, I registered my research topic and presented my research proposal investigating the analgesic potential of betanin in a murine inflammatory pain model, with a focus on its anti-inflammatory, antioxidant, and pain-related miRNA regulatory effects.

Research Proposal Seminar

I then began the practical phase of my research, implementing the proposed study design. This phase included animal purchase and acclimatization, induction of the pain model, treatment administration, behavioral assessments, sacrifice and dissection, sample collection, and subsequent molecular, biochemical and ultrastructural analyses, along with in silico simulations.

Induction of Pain and Peripheral Inflammation Assessment

Paw Assessment

Paw Thickness Measurement to Assess Peripheral Inflammation

Treatment Preparation

Betanin, Purchased from Sigma Aldrich

Treatment Doses Preparation

Dissection and Sample Collection

Sample Collection Day

Molecular & Biochemical Analysis
Molecular

Tissue Lysis Using Qiagen TissueLyser LT

miRNeasy Kit for miRNA Extraction, Purchased from Qiagen

Me Inside the Molecular Biology Lab! 😎

Purity Assessment and Concentration Determination Using NanoDrop2000

Reverse Transcription Using SimpliAmp Thermal Cycler

qRT-PCR Using Qiagen Rotor-gene Q

Biochemical

ELISA for Pro and Anti-Inflammatory Markers

While Waiting During the ELISA Incubation Periods! 🫠

Colorimetric Lipid Peroxidation Assessmnet

In silico Simulations

Molecular Docking Simulation

Data anlysis and representation.

Graphs, Scientific Illustrations, and TE Micrographs

And finally, publishing my article in Biomedicines, a Q1 journal (IF: 3.9, CS: 6.8), entitled:

Betanin from Beta vulgaris Attenuates Complete Freund’s Adjuvant-Induced Inflammatory Pain: Integrated Preclinical and In Silico Insights

At the end of this journey, and with all praise to Allah, I would like to sincerely acknowledge everyone who supported me throughout—my family, my supervisors, and my colleagues.

Stories | Ahmed Massoud

Snippets from My Master’s Journey

Throughout my master’s journey, I have been fortunate to grow academically and professionally. This page shares selected moments that shaped this experience.

Induction of Pain and Peripheral Inflammation Assessment

Treatment Preparation

Dissection and Sample Collection

Molecular & Biochemical Analysis

Molecular

Biochemical

In silico Simulations

Data anlysis and representation.

And finally, publishing my article in Biomedicines, a Q1 journal (IF: 3.9, CS: 6.8), entitled:

At the end of this journey, and with all praise to Allah, I would like to sincerely acknowledge everyone who supported me throughout—my family, my supervisors, and my colleagues.