Review Article | Ahmed Massoud

Integrating Single-Cell Sequencing and Proteomics to Unravel Stem Cell Heterogeneity: Implications for Precision Regenerative Medicine

Thu, 09 Apr 2026 00:00:00 +0000

Abstract

Stem cell populations are inherently diverse, consisting of distinct subtypes with unique molecular signatures, self-renewal capacities, and differentiation potentials. This natural variability complicates their clinical use, leading to unpredictable treatment outcomes and safety concerns in regenerative medicine. Traditional bulk analysis methods obscure this vital cellular diversity by providing average data. Therefore, studying stem cell heterogeneity at the single-cell level is crucial for developing reliable and precise therapeutic strategies. This review examines how integrating single-cell sequencing with proteomics technologies enhances the current understanding of stem cell biology. We discuss how single-cell RNA sequencing reveals transcriptomic diversity, identifies rare subpopulations, and traces lineage pathways. Additionally, we highlight emerging single-cell proteomic techniques, such as mass cytometry and high-sensitivity mass spectrometry, which directly analyze functional protein expression and signaling networks that ultimately define cellular phenotypes. Combining these methods provides a comprehensive view of stem cell states, offering insights into the molecular mechanisms that influence fate decisions and responses to external signals. Ultimately, these advances enable precision regenerative medicine by improving cell characterization and developing tailored differentiation strategies for safe and effective clinical applications.

Cite this article: Zayed, M., Massoud, A., Al-Haddad, R. et al. Integrating Single-Cell Sequencing and Proteomics to Unravel Stem Cell Heterogeneity: Implications for Precision Regenerative Medicine. Stem Cell Rev and Rep (2026).

Harnessing Adipose Ferroptosis: A Promising Novel Pathway for Obesity Treatment

Sat, 25 Oct 2025 00:00:00 +0000

Abstract

This review summarizes the potential of targeting ferroptosis-iron-dependent lipid peroxidation-as a novel strategy for treating obesity and its metabolic complications through mechanisms different from traditional interventions. Recent preclinical studies show that selectively inducing ferroptosis in lipid-rich adipocytes and obese mice can effectively decrease fat mass and enhance metabolic health. These ferroptosis-based methods target the fundamental cellular processes of adipocyte dysfunction, affecting lipid metabolism and reducing oxidative stress. The strategy appears promising in addressing major metabolic issues such as insulin resistance and hepatic steatosis. In addition, the ability to customize ferroptosis inducers based on individual metabolic profiles offers a pathway to highly personalized obesity treatments. Ferroptosis agonists offer a revolutionary therapeutic approach for obesity treatment by directly reducing fat mass and targeting key metabolic issues. However, applying these findings in clinical settings requires careful evaluation of the long-term safety and effects of pharmacological ferroptosis induction. This review highlights important gaps in current knowledge and suggests future research directions crucial for developing these innovative therapies.

Cite this article: Zayed, M., Massoud, A., Hassan, F. et al. Harnessing Adipose Ferroptosis: A Promising Novel Pathway for Obesity Treatment. Curr Obes Rep 14, 74 (2025).

Pharmacological modulation of ferroptosis: Preclinical promise, clinical facts, and translational challenges

Mon, 01 Jan 0001 00:00:00 +0000

Abstract

Article still under review.

Targeting mitochondrial dysfunction with engineered mesenchymal stem cell-derived extracellular vesicles for neurodegenerative therapies

Mon, 01 Jan 0001 00:00:00 +0000

Abstract

Article still under review.